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Continue to ChatPlainly, when perfusate from an organ or blood from an animal is used for superfusion, substances can reach the assay tissues within a few seconds of generation or release. - John Vane from https://whatsmyquote.com/quote/plainly-when-perfusate-from-an-organ-or-blood-from-an-animal-is-used-for-superfusion-substances-can-reach-the